Towards a CRISPeR understanding of homologous recombination with high-throughput functional genomics. Hayward, S.B., and Ciccia, A. (2021). Curr. Opin. Genet. Dev. 71, 171-181

REV1-Polζ maintains the viability of homologous recombination-deficient cancer cells through mutagenic repair of PRIMPOL-dependent ssDNA gaps. Taglialatela, A., Leuzzi, G., Sannino, V., Cuella-Martin, R., Huang, J.W., Wu-Baer, F., Baer, R., Costanzo, V., and Ciccia, A. (2021). Mol. Cell 81(19), 4008-4025.

                               Selected for the cover of Molecular Cell

Functional interrogation of DNA damage response variants with base editing screens. Cuella-Martin, R., Hayward, S.B., Fan, X., Chen, X., Huang, J.W., Taglialatela, A., Leuzzi, G., Zhao, J., Rabadan, R., Lu, C., Shen, Y., and Ciccia, A. (2021). Cell 184(4), 1081-1097.


Highlights: CRISPR base editor screens identify variant function at scale. Parrish, P.C.R., and Berger, A.H. (2021).

Mol. Cell 81(4), 647-648; Finding function with base editing screens. Koch, L. (2021). Nature Reviews Genetics



HATtracting nucleases to stalled forks. Leuzzi, G., Taglialatela, A., and Ciccia, A. (2020). Mol. Cell 80(2), 177-180.

Time for remodeling: SNF2-family DNA translocases in replication fork metabolism and human disease. Joseph, S.A., Taglialatela, A., Leuzzi, G., Huang, J.W., Cuella-Martin, R., and Ciccia, A. (2020). DNA Repair 95, 102943.

MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage. Huang, J.W., Taglialatela, A., Acharya, A., Leuzzi, G., Nambiar, T.S., Cuella-Martin, R., Hayward, S.B., Joseph, S.A., Brunette, G.J., Anand, R., Soni, R.K., Clark, N.L., Bernstein, K.A., Cejka, P., and Ciccia, A. (2020). Nature Comm. 11, 2948.

Detection of marker-free precision genome editing and genetic variation through the capture of genomic signatures. Billon, P., Nambiar, T.S., Hayward, S.B., Zafra, M.P., Schatoff, E.M., Oshima, K., Dunbar, A., Wong, M., Breinig, M., Park, Y.C., Tschaharganeh, D.F., Levine, R.L., Baer, R., Ferrando, A.A., Dow, L.E., and Ciccia, A. (2020). Cell Reports 30(10), 3280-3295.


Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant.  Nambiar, T.S., Billon, P., Diedenhofen, G., Hayward, S.B., Taglialatela, A., Cai, K., Huang, J.W., Leuzzi, G., Cuella-Martin, R., Palacios, A., Gupta, A., Egli, D., and Ciccia, A. (2019). Nature Comm. 10, 3395.


The BRCT domains of the BRCA1 and BARD1 tumor suppressors differentially regulate homology-directed repair and stalled fork protection. Billing, D., Horiguchi, M., Wu-Baer, F., Taglialatela, A., Leuzzi, G., Alvarez, S., Jiang, W., Zha, S., Szabolcs, M., Lin, C.-S., Ciccia, A., and Baer, R.  (2018). Mol. Cell 72(1), 127-139.


Restoration of replication fork stability in BRCA1- and BRCA2-deficient cells by inactivation of SNF2-family fork remodelers. Taglialatela, A., Alvarez, S., Leuzzi, G., Sannino, V., Ranjha, L., Huang, J.W., Madubata, C., Anand, R., Levy, B.,  Rabadan, R., Cejka, P., Costanzo, V., Ciccia, A.  (2017). Mol. Cell 68(2), 414-430.

CRISPR-mediated base editing enables efficient disruption of eukaryotic genes through induction of STOP codons. Billon, P., Bryant, E.E., Joseph, S.A., Nambiar, T.S., Hayward, S.B., Rothstein, R., Ciccia, A.  (2017). Mol. Cell 67(6), 1068-1079.

Replication fork slowing and reversal upon DNA damage require PCNA polyubiquitination and ZRANB3 DNA translocase activity. Vujanovic, M., Krietsch, J., Raso, M.C., Terraneo, N., Zellweger, R., Schmid, J.A., Taglialatela, A., Huang, J.W., Holland, C.L., Zwicky, K., Herrador, R., Jacobs, H., Cortez, D., Ciccia, A., Penengo, L., Lopes, M.  (2017). Mol. Cell 67(5), 882-890.

SMARCAL1-mediated fork reversal triggers MRE11-dependent degradation of nascent DNA in the absence of BRCA2 and stable RAD51 nucleofilaments. Kolinjivadi, A.M., Sannino, V., De Antoni, A., Zadorozhny, K., Kilkenny, M., Técher, H., Baldi, G., Shen, R., Ciccia, A., Pellegrini, L., Krejci, L., Costanzo, V. (2017). Mol. Cell 67(5), 867-881.


Stressing out about RAD52. Ciccia, A.* and Symington, L.S.* (2016). Mol. Cell 64(6), 1017-1019.

* Corresponding authors

A systematic analysis of factors localized to damaged chromatin reveals PARP-dependent recruitment of transcription factors. Izhar, L., Adamson, B., Ciccia, A., Lewis, J., Pontano-Vaites, L., Leng, Y., Liang, A.C., Westbrook, T.F., Harper, J.W., and Elledge, S.J. (2015). Cell Reports 11(9), 1486-1500.


Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response. Ciccia, A.*, Huang, J.W., Izhar, L., Sowa, M.E., Harper, J.W., and Elledge, S.J.* (2014). Proc. Natl. Acad. Sci U S A 111, 18631-18636.

* Corresponding authors


Protein interaction discovery using parallel analysis of translated ORFs (PLATO). Zhu, J., Larman, H.B., Gao, G., Somwar, R., Zhang, Z., Laserson, U., Ciccia, A., Pavlova, N., Church, G., Zhang, W., Kesari, S., and Elledge, S.J. (2013). Nat. Biotechnol. 31(4), 331-334.


Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress. Ciccia, A., Nimonkar, A.V., Hu, Y., Hajdu, I., Achar, Y.J., Izhar, L., Petit, S.A., Adamson, B., Yoon, J.C., Kowalczykowski, S.C., Livingston, D.M., Haracska, L., and Elledge, S.J. (2012). Mol. Cell 47(3), 396-409.


Wolf-Hirschhorn syndrome candidate 1 is involved in the cellular response to DNA damage. Hajdu, I., Ciccia, A., Lewis, S.M., and Elledge, S.J. (2011). Proc. Natl. Acad. Sci. U S A 108(32), 13130-13134.


Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyclosome substrate. Emanuele, M.J., Ciccia, A., Elia, A.E., and Elledge, S.J. (2011). Proc. Natl. Acad. Sci. U S A 108(24), 9845-9850.


Autoantigen discovery with a synthetic human peptidome. Larman, H.B., Zhao, Z., Laserson, U., Li, M.Z., Ciccia, A., Gakidis, M.A., Church, G.M., Kesari, S., Leproust, E.M., Solimini, N.L., Elledge, S.J. (2011). Nat. Biotechnol. 29(6), 535-541.


The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo. Meerbrey, K.L., Hu, G., Kessler, J.D., Roarty, K., Li, M.Z., Fang, J.E., Herschkowitz, J.I., Burrows, A.E., Ciccia, A., Sun, T., Schmitt, E.M., Bernardi, R.J., Fu, X., Bland, C.S., Cooper, T.A., Schiff, R., Rosen, J.M., Westbrook, T.F., Elledge, S.J. (2011). Proc. Natl. Acad. Sci. U S A 108(9), 3665-3670.


A genome-wide camptothecin sensitivity screen identifies a mammalian MMS22L-NFKBIL2 complex required for genomic stability. O'Connell, B.C., Adamson, B., Lydeard, J.R., Sowa, M.E., Ciccia, A., Bredemeyer, A.L., Schlabach, M. Gygi, S.P., Elledge, S.J., and Harper, J.W. (2010). Mol. Cell 40(4), 645-657.


The DNA damage response: making it safe to play with knives. Ciccia, A., and Elledge, S.J. (2010). Mol. Cell 40(2) 179-204.


The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart. Ciccia, A., Bredemeyer, A.L., Sowa, M.E., Terret, M.E., Jallepalli, P.V., Harper,  J.W., and Elledge, S.J. (2009). Genes Dev. 23(20), 2415-2425. 


FANCM and FAAP24 function in ATR-mediated checkpoint signaling independently of the Fanconi anemia core complex. Collis, S.J, Ciccia, A., Deans, A.J., Horejsi, Z., Martin, J.S., Maslen, S.L., Skehel, J.M. Elledge S.J., West, S.C. and Boulton, S.J. (2008). Mol. Cell 32(3), 313- 324.


Structural and functional relationships of the XPF/MUS81 family of proteins. Ciccia, A., McDonald, N., and West, S.C. (2008). Annu. Rev. Biochem. 77, 259-287.


Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM. Ciccia, A., Ling, C., Coulthard, R., Yan, Z., Xue, Y., Meetei, A.R., Laghmani el, H., Joenje, H., McDonald, N., de Winter, J.P., Wang, W., and West, S.C. (2007). Mol. Cell 25(3), 331-343. 


EME1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells. Abraham, J, Lemmers, B., Hande, M. P., Moynahan, M. E., Chahwan, C., Ciccia, A., Essers, J., Hanada, K., Chahwan, R., Khaw, A. K., McPherson, P., Shehabeldin, A., Laister, R., Arrowsmith, C., Kanaar, R., West, S.C., Jasin, M., Hakem, R.  (2003). EMBO J. 22, 6137-6147.


Identification and characterization of the human MUS81-EME1 endonuclease. Ciccia, A., Constantinou, A., and West, S. C (2003). J. Biol. Chem. 278(27), 25172-25178.